For the longest time people have been exploring the frontiers of science and technology to look for materials and means to maintain health, to prevent/treat diseases, and to live a long, productive life. The key to reaching these goals lies in the regeneration of tissues and organs in the face of aging, infection of pathogens, and environmental insults. Regeneration maintains or restores the original, physiological structure and function of the tissues and organs in the body. Through 20 years of basic research and clinical practice, Dr. Xu has led a team of scientists and clinicians to significantly shorten the distance between these dreams and the reality in the regeneration of human tissues and organs. Starting with regeneration of skin, the largest organ of the body, Dr. Xu has established an innovative medical and nutritional system for enabling the body to regenerate and repair itself so as to maximize its lifespan and to allow us to live a healthy and productive life.
I. Organ Regeneration under Condition Provided by Using MEBO¡¯s Regenerative System Recapitulates Embryonic Development
Critical to the health of a life is to ensure the health of the cell, the basic unit of life. During the clinical studies of wound healing of adult burn patients, Dr. Xu discovered that in the presence of MEBO wound ointment somatic cells in the wounds are activated or induced to behave like embryonic stem cells, actively proliferating and differentiating directionally to produce a completely normal and healthy skin organ, including all appendages and with the correct physiological structure and function.
• Regeneration of Skin with Normal Physiological Structure and Function
Figure 1 shows a typical process of wound healing and skin regeneration of patients treated with MEBO¡¯s regenerative system. Compared to conventional burn therapy which would require skin grafting for extensive, deep burns, and often cannot avoid disfiguring scarring and disablement, Dr. Xu¡¯s approach potentiates and maximizes the ability of the body to heal itself by using MEBO¡¯s regenerative system (which includes applications of the wound ointment and unique techniques of wound management) to maintain the burns wound in an optimum, physiologically moist environment and to supply vital nutrients to enhance the regenerative ability of the residual viable tissue. Based on extensive preclinical and clinical research that produced evidence showing the dynamic changes of cells at both the cellular and tissue levels for adult humans undergoing wound healing under the influence of MEBO wound ointment, Dr. Xu and his research team have shown that the regeneration of skin under the conditions provided by using MEBO¡¯s regenerative system recapitulates embryonic development and mimics scar-less wound healing of a fetus.
Dr. Xu and his research team demonstrated for the first time that embryonic epidermal stem cells are induced or activated and able to proliferate in adult humans under conditions favorable for physiological tissue repair and organ regeneration. Such regenerative conditions are provided by applying MEBO wound ointment topically to the wounds and using unique techniques of wound management.
• Embryonic Epidermal Stem Cells Detected in Wounds Treated by Using MEBO¡¯s Regenerative System
During the treatment of the patients with at least deep partial thickness burns (that would normally require skin grafting if treated with conventional burn therapy), vigorous cellular activity on the wounds was observed in the presence of MEBO wound ointment. These ¡°hyper-active¡± cells are confirmed to be cells expressing keratin-19 (K-19) detected by immunofluorescent staining with a monoclonal antibody against human K-19. Figure 2 showing dynamic changes in the level of K-19 expressing regenerative cells monitored at different time points during wound healing of a patient under the treatment of MEBO/MEBT.
II. Regeneration of Damaged or Lost Limbs by Using MEBO¡¯s Regenerative System
Consistent with the regeneration of functional skin organ of an adult, application of MEBO/MEBT to human adult limb regeneration has produced groundbreaking clinical results. The clinical evidence collected from near a thousand cases shows that after treated with MEBO wound ointment adult patients with damaged or lost limbs can recover with fingertips regenerated with normal physiological structure and function.
• MEBO Proves that Regenerative Potential of Adult Humans is Much Stronger than Previously Expected
It has been well established that vertebrates of lower species, particularly urodele amphibians (common called ¡°salamanders¡±), have an intrinsic capacity to regenerate a variety of body parts, including limbs, tail, jaw, and retina. In higher mammals, examples of complex tissue regeneration are less common but can be seen in the seasonal regrowth of deer antlers. For humans, there is description of distal fingertip regeneration in children, but not in fully developed adults. Some concluded that regenerative potential declines with the evolution of complexity. As here, application of MEBO/MEBT to lost or severely damaged fingers of adult humans could not only repair the wounds but also regeneration of fingers with normal structure and function, including bones, muscles, nerves, blood vessels, connective tissue, finger nails and skin. These results demonstrate that the regenerative potential of adult humans is much stronger than previously expected. Consistent with what was observed for the regeneration of skin of burn patients, establishing an appropriate environment favoring regeneration and providing materials to the wound to stimulate and sustain the regeneration is the key to the success.
III. Certain Somatic Cells in Every Tissue or Organ of the Body are Potential Regenerative Cells
At the cellular level, it was found that ordinary adult somatic cells isolated from animals could be induced to behave like stem cells under the conditions provided by the MEBO regenerative substances in cell culture. More surprisingly, the cells proliferated and differentiated orderly to form tissue with substantially the same physiological structure and function as the corresponding tissue in vivo and in situ. To differentiate such cells from normal adult stem cells such as basal epidermal stem cells, Dr. Xu coined these cells ¡°potential regenerative cells¡± (PRCs).
• Comparing PRCs with induced Pluripotent Stem Cells (iPS Cells)
Based on further cellular, animal experiments and clinical studies, Dr. Xu proposed that PRCs exist in virtually every tissue and organ of the body and may not need specific ¡°niches¡± to be tucked away. Unlike the iPS cells produced by inserting exogenerous genes in order to maintain an undifferentiated state (see review by Yu & Thomson (2008) Genes & Development 22:1987-1997), the native PRCs existing in the body need not to be genetically engineered in order to be activated or induced to behave like stem cells. Unlike the iPS cells, the PRCs in the presence of the MEBO regenerative substances do not differentiate chaotically to form tumor-like cell masses. Instead, the differentiation of PRCs follows a lineage specific to the site of the body from which the PRCs are originally isolated. Such a path is clearly illustrated by the formation of the intestinal villi from small intestinal cells isolated from normal human intestines. In the presence of the MEBO regenerative substances, single, isolated adult intestinal cells proliferated and directionally differentiated to epithelial cells, goblet cells, Paneth cells, and endocrinal cells, which adhered to each other to form the distinct brush-like structure of intestinal villi in the cell culture. Similar formation of physiological tissues from PRCs from various tissues and organs, such as heart, lung, liver, pancreas, bone marrow, and nerves, has been observed in the cell culture in the presence of MEBO.
More significantly, a series of products based on MEBO regenerative substances have been and are being developed to promote tissue and organ regeneration in the body. For example, an oral formulation based on the MEBO regenerative substances has been clinically tested and found to be able to repair and regenerate the mucosal lining of the gastrointestinal tract without scar formation, which is consistent with the regeneration of skin using MEBO/MEBT.
IV. MEBO regenerative substances selectively inhibit cancer cell growth while promote regeneration of normal tissue
Having proven that the MEBO regenerative substances are able to stimulate and sustain vigorous growth and directional differentiation of healthy human cells, Dr. Xu asked the converse question: ¡°How do MEBO regenerative substances affect cancer cells?¡¯ A series of cellular and animal experiments have been designed to test the effects of MEBO on cancer cells in vitro and in vivo. It has been demonstrated that not only cancer cells, but also immortalized cells transformed using virus, are not able to sustain their growth in the presence of the MEBO regenerative substances; instead, they undergo a growth arrest which is followed by cell death eventually. Controlled cellular studies showed that healthy human cells thrive, while cancer cells from the same organ stop growing, in the presence of the same reagent containing the MEBO regenerative substances. Consistent with the results obtained from the cell culture experiments, the cells in human tumor tissues cultured in vitro also stop growing, leading to a disintegration of the tumor tissues. Such unique dual functions of MEBO open the door to future cancer prevention and treatment that could circumvent common problems associated with conventional cancer therapy, such as adverse cytotoxicity for healthy cells in the body and compromised immune system.
Currently, clinical studies of terminal cancer patients are undergoing in China to test the ability of the MEBO regenerative substances to combat cancer and re-establish the homeostasis of the body by stimulating the growth of residual healthy progenitor or stem cells to regenerate tissues/organs, and by halting the growth of transformed or malignant cancer cells so as to eradicate cancer cells and tumors eventually. Preliminary encouraging results were obtained from the first phase of this study of terminal cancer patients who have failed medical cancer treatment, and whose cancer includes the most common and lethal ones: lung cancer, liver cancer, gastrointestinal cancer, breast cancer, prostate cancer, ovarian cancer, brain tumors, and leukemia. Among the 204 patients who have participated in the treatment with MEBO¡¯s innovative total nutritional therapy (that utilizes the MEBO regenerative substances) for more than 4 months, those who started at a relatively stabilized stage have a survival rate of 100% (16/16); those at a stage of disease progression have a survival rate of 61.54 % (77/125) and are currently stabilized; and those at a stage of end-of-life salvage have a survival rate of 33.33% (20/63). These data are distinctively different from the half-month survival rates of end-of-life or terminal cancer patients under the international cancer treatment standards. In this study there is a significant increase not only in the overall survival rate, but also in the survival rate separately for various stages and types of cancer. These preliminary results from the clinical study bring hope to us in our fight against cancer.
By harnessing the natural power of our body to regenerate or heal itself, MEBO is also developing additional MEBO regenerative medicines and nutrients that will significantly impact human health, including remedies for preventing or treating neurodegenerative diseases, diabetes, and other diseases associated with organ degeneration and failure. If successful, we believe that these new technologies and approaches may allow us to expand our life expectancy and live a more healthy and productive life.